This study focuses on characterization of Emerging Organic Contaminants (EOCs) in the Awash River basin. Characterization of the EOCs was supplemented by chemical analysis of samples from river, boreholes, tap water, and surface water reservoirs. Analyses of environmental isotopes (δ 2 H, δ 18 O, and 222Rn) were used to investigate the exchange of contaminants between surface and groundwater supply sources. The analysis showed new types of contaminants in the water supply sources with potential impact on human and wider environmental health.